Tissue distribution of Lkn-1, CKβ8-1, and CKβ8 mRNA. The human multiple tissue blot (first panel) was purchased from Clonetech. Each oligonucleotide probe (5 × 10⁶ cpm/mL) was hybridized to the membrane at 45°C and finally washed with 2 × SSC at 45°C for 20 minutes. Indicated by arrows are CKβ8-1, CKβ8, and Lkn-1 mRNAs.