American Society of Hematology

Fig. 2.

$Fig. 2. RT-PCR analysis of amphotropic retrovirus receptor (Amphotropic R) and Gibbon Ape Leukemia Virus receptor (GALV R) mRNA levels in human fetal liver and adult BM. RNA was extracted from unfractionated (UNFR), HSC-enriched Lin−CD34+ CD38−, and progenitor-enriched Lin− CD34+ CD38+ fetal liver and adult BM cells. The levels of Amphotropic R (upper bands) and GALV R mRNA (middle bands) were quantified based on the level of β2-microglobulin mRNA (lower bands) in the same sample. These values were then compared with the levels of Amphotropic R or GALV R mRNA in HeLa cells (right column) and compared with the mRNA levels in unfractionated control BM (see Table 2). Adult human BM cells were fractionated by CCE at flow rates (FR) of 20, 25, 30, 35, and 40 mL/min (FR20 to FR40). Samples from five separate experiments were analyzed (see Table 2).$

RT-PCR analysis of amphotropic retrovirus receptor (Amphotropic R) and Gibbon Ape Leukemia Virus receptor (GALV R) mRNA levels in human fetal liver and adult BM. RNA was extracted from unfractionated (UNFR), HSC-enriched Lin⁻CD34⁺ CD38⁻, and progenitor-enriched Lin⁻ CD34⁺ CD38⁺ fetal liver and adult BM cells. The levels of Amphotropic R (upper bands) and GALV R mRNA (middle bands) were quantified based on the level of β2-microglobulin mRNA (lower bands) in the same sample. These values were then compared with the levels of Amphotropic R or GALV R mRNA in HeLa cells (right column) and compared with the mRNA levels in unfractionated control BM (see Table 2). Adult human BM cells were fractionated by CCE at flow rates (FR) of 20, 25, 30, 35, and 40 mL/min (FR20 to FR40). Samples from five separate experiments were analyzed (see Table 2).

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