Fig. 6.
![Fig. 6. Regulation of the p45 transactivation domain by A-kinase in MEL cells. MEL cells were cotransfected with 300 ng of pGAL4-Luc, 300 ng of pRSV-βGal, the indicated amounts of transactivator plasmid containing the N-terminal 83 amino acids of p45 [pGal4/p45(Δ83)], and 200 ng of either A-kinase C-subunit expression vector (•) or empty vector (○) as described in Materials and Methods. Luciferase activity was normalized to β-galactosidase activity; results represent the mean ± SD of three independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/9/10.1182_blood.v91.9.3193/3/blod40916006x.jpeg?Expires=1722710242&Signature=xFphqZHCxJxjFkj47sVtQ-rdqpao0wcNbcWZUmEujwgndnJOuCpWh83zUiZRfYnpraDXQzM2bsoyAPYhxwdZ1jU4Fy6iVDDDAiw8eDcdlV2-Z-16NUzhraX29z~JwTBwbmvwHLVpq5X9ucan0Is4mARmadDtOEnHi2suewDifs1DSLxvrhA3UelLCt-GR2ssbOIRNhp4pmfXvFdrr9jEY3Y3yM4VHm4qTZOc1sN1G1R~XiYbuKxT0CkvCw94CwpoLp91XjI5H2taFsgdtYFR6dpfZMLdn2Vykhtmytq9ue74QMnsA797RoHPiBr-aQmNLYWjd~0nvdcMmiSOlREGFA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Regulation of the p45 transactivation domain by A-kinase in MEL cells. MEL cells were cotransfected with 300 ng of pGAL4-Luc, 300 ng of pRSV-βGal, the indicated amounts of transactivator plasmid containing the N-terminal 83 amino acids of p45 [pGal4/p45(Δ83)], and 200 ng of either A-kinase C-subunit expression vector (•) or empty vector (○) as described in Materials and Methods. Luciferase activity was normalized to β-galactosidase activity; results represent the mean ± SD of three independent experiments.
