The phenotype of DC generated with or without LPS. DC were cultured for 5 days in the absence (standard lines) or presence of LPS (bold lines). Day-5 DC were analyzed by flow cytometry for the surface expression of the antigens indicated using the antibodies listed in Materials and Methods. Cells were analyzed without scatter gating. In the first histogram, forward scatter (FSC, x-axis) is plotted against the number of events (y-axis).