Fig. 7.
![Fig. 7. LLnL does not inhibit tyrosine phosphatase activity. Growing Ba/F3 cells were washed and lysed with NP-40 buffer in the absence of vanadate. Lysate from 105 cells was assayed for total tyrosine phosphatase activity. LLnL (▨) (50 μmol/L) or DMSO (□) as carrier were added to the reaction at a final concentration identical to that used when added to cell cultures. As a positive control, orthovanadate (▧) (1 mmol/L) was also added to inhibit phosphatase activity. Data were normalized to activity obtained from control reactions (no additions [▪]) and each bar represents the average of duplicate samples taken from a single experiment. Identical results were obtained from several experiments using different dilutions of lysate.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/9/10.1182_blood.v91.9.3182/3/blod40912007y.jpeg?Expires=1722702748&Signature=P8BCgg2~iyWF0QeRuNPzzW6pviHWV4RITydBZ~adAYiHGH54wBEDkKGGwTVHvDtc8lSWjaFA6ncFaxC6~xzPmlf4qI1WkqfTXNjoj9IIQW3c9PVQcZCTwAl2esPWbLhdwXn80xGQtypONrhDq5GCZO96CJRvNKuxu~XNUwOAvYRPDg3Q8jJ0H4yO35vQ7L8cPEWzECJ8LyipCU~L37MkGUWD-w0RcpkRevMhdHxfC-7MXJvROo6duVnEP8ka4gz9fCZEVGEkzwZpE2f~6QUtQ5a4gkDMBru1C5-7vOb6A1atr6YnTpBnCPpn1TNbm8-KkWoQ9mmg8oRL5sqHTx5zMw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
LLnL does not inhibit tyrosine phosphatase activity. Growing Ba/F3 cells were washed and lysed with NP-40 buffer in the absence of vanadate. Lysate from 105 cells was assayed for total tyrosine phosphatase activity. LLnL (▨) (50 μmol/L) or DMSO (□) as carrier were added to the reaction at a final concentration identical to that used when added to cell cultures. As a positive control, orthovanadate (▧) (1 mmol/L) was also added to inhibit phosphatase activity. Data were normalized to activity obtained from control reactions (no additions [▪]) and each bar represents the average of duplicate samples taken from a single experiment. Identical results were obtained from several experiments using different dilutions of lysate.
