Fig. 5.
Fig. 5. (A) A total of 2.5 × 104CD34+ cells isolated from cord blood were placed in triplicate into wells precoated with H120, H0, or BSA containing serum-free medium and standard growth factors. Adherent and nonadherent cells were counted after 6 days. Hatched areas represent the adherent cell component. (B) A total of 5 × 103CD34+ cells were placed in triplicate into wells precoated with H120 fragment or left uncoated, containing serum-free medium and the standard concentration of growth factors. Adherent and nonadherent cells were counted after 8 days and analyzed by FACS for the proportion of CD34+ cells in each fraction. Hatched areas represent the adherent cell component.

(A) A total of 2.5 × 104CD34+ cells isolated from cord blood were placed in triplicate into wells precoated with H120, H0, or BSA containing serum-free medium and standard growth factors. Adherent and nonadherent cells were counted after 6 days. Hatched areas represent the adherent cell component. (B) A total of 5 × 103CD34+ cells were placed in triplicate into wells precoated with H120 fragment or left uncoated, containing serum-free medium and the standard concentration of growth factors. Adherent and nonadherent cells were counted after 8 days and analyzed by FACS for the proportion of CD34+ cells in each fraction. Hatched areas represent the adherent cell component.

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