Primarsequence and cytogenetic location of 16 microsatellite markers used in the study. The distance in centimorgans between the markers is indicated. The PCR conditions included denaturation in 95° for 5 minutes. Denaturation temperature was always 94°C for 30 to 60 seconds. Annealing time was 30°C and the temperature is shown for every microsatelite marker. Elongation temperature was 72°C for 30 to 40 seconds. The number of cycles is shown for every marker. Amplification was always concluded with 10-minute final extension at 72°C. Marker D6S15433 and D6S1709, located between D6S468 and D6S283, and marker D6S434 and D6S1028, located between D6S283 and D6S301, are not shown in the figure.