![Fig. 7. Regulation of RARα and PML-RARα by ATRA. (A) The indicated cell types were treated with vehicle control (ethanol [−]) or 10−6 mol/L ATRA (+) for 96 hours; total cellular protein was obtained, and equal amounts were electrophoresed and immunoblotted successively with RARα (top panel) and actin (bottom panel) antibodies. Molecular weight markers are indicated to the left of the gel (in kilodaltons × 10−3). The migration of the PML-RARα L isoform (L), PML-RARα S isoform (S), and endogenous RARα (E) are indicated. (B) Quantitation of the results shown in (A) including data from additional independent experiments. The control level of expression of either endogenous RARα or PML-RARα was set at 100% in each case, and the relative level of expression of these proteins in response to ATRA was quantitated by densitometric analysis of Western blots such as those shown in (A). Values were corrected for differences in loading and transfer by incorporating the actin control in the calculation.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/9/10.1182_blood.v91.9.3347/3/blod40928007w.jpeg?Expires=1722709837&Signature=NDo-ddhOVN-lUyKwBOmM3V2W3a8DvplXqMka8ykGDxeJWI4c7XV8Jx1lHpQIhIPmWjftHyEHZlWRMv1f29l7lt64-oHXoLe-5zpcIssCrWWdYSOi7DDQ52fcb6rgRZHUr4EkyzfXaLu8xaeFEEVsUe6wUZo8JhZo6PC8Q5uAUyC4KchNPKmBlxCLmJtQz4b0Yeevq7BeB95wPhs2D60F9FfG3MiCncg4ljXbHVPSSj7FYWxjsEyNGeJRsBoEIrd-ALaU02biVFesj2xurGRVYOdXIdNgn-6EIVeScJ3gp4LEGJQZMsmK8vSldmyZm7dz6cFSxABXpxgEz6mrY169Yw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Regulation of RARα and PML-RARα by ATRA. (A) The indicated cell types were treated with vehicle control (ethanol [−]) or 10−6 mol/L ATRA (+) for 96 hours; total cellular protein was obtained, and equal amounts were electrophoresed and immunoblotted successively with RARα (top panel) and actin (bottom panel) antibodies. Molecular weight markers are indicated to the left of the gel (in kilodaltons × 10−3). The migration of the PML-RARα L isoform (L), PML-RARα S isoform (S), and endogenous RARα (E) are indicated. (B) Quantitation of the results shown in (A) including data from additional independent experiments. The control level of expression of either endogenous RARα or PML-RARα was set at 100% in each case, and the relative level of expression of these proteins in response to ATRA was quantitated by densitometric analysis of Western blots such as those shown in (A). Values were corrected for differences in loading and transfer by incorporating the actin control in the calculation.
