FACS analysis of gp130 on thymocytes and splenic T cells from C57BL/6 mice. Freshly isolated thymocytes with CD4⁻/CD8⁻, CD4⁺/CD8⁺, CD4⁺/CD8⁻, or CD4⁻/CD8⁺ phenotypes and splenic CD4⁺ or CD8⁺ T cells from C57BL/6 mice (12-week-old female) were analyzed for gp130 expression. Closed and open histograms show the staining with RX19 and control rat myeloma IgG_2a, respectively.