Fig. 2.
Fig. 2. Recognition of murine gp130 by MoAb RX19 was not inhibited by the complex of IL-6 and sIL-6R. Thymocytes from C57BL/6 mice (12-week-old female) were incubated in PBS, 5 mmol/L EDTA, PH7.2, 0.5% BSA, with or without a combination of IL-6 and sIL-6R at 37°C for 20 minutes, the cells were then stained with RX19 (closed histograms) or control rat myeloma IgG2a (open histograms).

Recognition of murine gp130 by MoAb RX19 was not inhibited by the complex of IL-6 and sIL-6R. Thymocytes from C57BL/6 mice (12-week-old female) were incubated in PBS, 5 mmol/L EDTA, PH7.2, 0.5% BSA, with or without a combination of IL-6 and sIL-6R at 37°C for 20 minutes, the cells were then stained with RX19 (closed histograms) or control rat myeloma IgG2a (open histograms).

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