AML1-ETO expression in the dysplastic hematopoietic cells. Western blot analysis of total cell lysates prepared from wild-type (Wt.) or AML1-ETO/+ knock-in (KI) cells. Cell lysates transferred to nitrocellulose membranes were blotted with affinity purified ETO N-terminal peptide antisera and visualized with supersignal ULTRA chemiluminescence substrate.