Fig. 2.
RT-PCR analysis of Lck expression in B-CLL. (A) Ethidium bromide staining of the amplification products of an Lck-specific first-strand cDNA retrotranscribed from the mRNA from either CLL B cells (lanes 3 and 4) or control T cells (lane 2) or THP-1 cells (lane 6). PCR amplification of the same region of the Lck coding sequence from a pGEX-Lck construct is shown (lane 1). Lane 2, molecular-weight marker. (B) Phosphorimager print of a Southern blot hybridization of the same samples with 32P-labeled Lck cDNA.

RT-PCR analysis of Lck expression in B-CLL. (A) Ethidium bromide staining of the amplification products of an Lck-specific first-strand cDNA retrotranscribed from the mRNA from either CLL B cells (lanes 3 and 4) or control T cells (lane 2) or THP-1 cells (lane 6). PCR amplification of the same region of the Lck coding sequence from a pGEX-Lck construct is shown (lane 1). Lane 2, molecular-weight marker. (B) Phosphorimager print of a Southern blot hybridization of the same samples with 32P-labeled Lck cDNA.

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