American Society of Hematology

Fig. 3.

$Fig. 3. Retinoid-receptor profile of RPMI 8226 cell line. Cells were treated or not for 7 hours with 10−7 mol/LatRA in defined medium. Total RNA (1 μg) was submitted to RT and PCR (for 15, 20, 25, 30, or 35 cycles), fractionated on 2% agarose gel, transferred to a Hybond N+ membrane, and hybridized to 32P-labeled oligonucleotidic probes for human RARα, RARβ, RARγ, RXRα, RXRβ, RXRγ, or β-actin. (For primers and probes see Materials and Methods).$

Retinoid-receptor profile of RPMI 8226 cell line. Cells were treated or not for 7 hours with 10⁻⁷ mol/LatRA in defined medium. Total RNA (1 μg) was submitted to RT and PCR (for 15, 20, 25, 30, or 35 cycles), fractionated on 2% agarose gel, transferred to a Hybond N⁺ membrane, and hybridized to ³²P-labeled oligonucleotidic probes for human RARα, RARβ, RARγ, RXRα, RXRβ, RXRγ, or β-actin. (For primers and probes see Materials and Methods).

This Feature Is Available To Subscribers Only