Adhesion of neutrophils to IC-bearing and IL-1 activated (30 ng/mL for 4 hours) or resting endothelial cells under nonstatic conditions. The role of L-selectin and E-selectin in mediating neutrophil adhesion to IC-bearing, IL-1-activated endothelial cells was determined by preincubating neutrophils and endothelial cells for 30 minutes at 4°C with the indicated MoAbs diluted at 10 μg/mL. The adhesion assay was performed under rotation (64 rpm) for 10 minutes at 37°C, keeping the concentration of MoAbs at 10 μg/mL. Experiments with resting, IC-bearing, and IL-1-activated endothelial cells (first three bars from the bottom) were performed. Control experiments contained buffer instead of MoAbs (Buffer). Values are expressed as mean ± SEM of three experiments; *P < .0001 for MoAbs v buffer.