Fig. 6.
![Fig. 6. Inhibition of VEGF expression by a catalytically inactive mutant Akt [HA-Akt(K179M)] in Ha-ras–transformed cells (NIH3T3R). NIH3T3R cells were cotransfected with HA-Akt(K179M) (5 μg) plasmid and a constant amount of reporter plasmid (2.5 μg; 1,511 bp; 385 bp; HIF-1 wt), and then exposed to hypoxia before assaying for luciferase activity. Values represent the means of at least three independent transfections. Error bars represent 1 SD of the mean.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/9/10.1182_blood.v90.9.3322/4/bl_0055f6.jpeg?Expires=1724253860&Signature=ZAb27QIhzi73CJ6TWkoo1i-UNI4tmwk1~8QRRqMsEVKfXXNGHZtSMGxaAZRzvR20SukQbpzXwklFKQ~RlPqsURdxqsFtbp9pTVEKD~iO6u1ZYLLywioqREnrJsbv8iwi8YeQ7PWCDd23EDs2Cb3ZpfWGdRZg0nt0oUa3aiNUY9qLroxi8wncHTdMTbAvw8dFGu~KdmcDQ0cY~YstQ9tZ62mslffURwTIN-j3sIjO8cAiVywl8Dkbk-8EWn0Jsmzk~uGixDsMFwPeux4W2qsyZQSoUupuYmM~4F3ATcAaUfDTrT2PBBo8hJ-JU17Nr9E4r3BKkC1MJZiBNMPTl1bMaA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Inhibition of VEGF expression by a catalytically inactive mutant Akt [HA-Akt(K179M)] in Ha-ras–transformed cells (NIH3T3R). NIH3T3R cells were cotransfected with HA-Akt(K179M) (5 μg) plasmid and a constant amount of reporter plasmid (2.5 μg; 1,511 bp; 385 bp; HIF-1 wt), and then exposed to hypoxia before assaying for luciferase activity. Values represent the means of at least three independent transfections. Error bars represent 1 SD of the mean.
