AC-derived DCs display a higher T-cell activation capacity than CD34⁺AC-derived DCs when growing in the same cytokine conditions. Graded numbers of AC-derived DCs generated after 7 days of culture in the presence of GM-CSF and IL-4 with (▴) or without (▵) TNF-α and CD34⁺AC-derived DCs generated after 14 days of culture in the presence of GM-CSF/IL-4/TNF-α (▪) were used in MLR as stimulator cells for 1.5 × 10⁵ thawed allogenic T cells. Cells were pulsed with ³H-TdR for 12 hours after 5 days of coculture. Data are expressed as means of sextuplet cultures (one of two experiments). ³H-TdR incorporation rates of irradiated DCs or purified T cells were always less than 900 cpm.