Indirect immunofluorescence and flow cytometric analysis of the surface expression of α4 and β1 integrins on rat leukocytes. (A) Representative histograms of rat neutrophil staining with TA-2 and Ha2/5 as compared with IgG1 or IgM control, respectively. (B) Actual fluorescence intensity (FI) values for α4 and β1 staining on neutrophils with MoAb TA-2 and Ha2/5 for six rats. *Mean FI values are significantly (P < .05) increased over mean FI values for IgG1 or IgM controls. (C) α4 and β1 expression was examined on neutrophils (PMN, ▪), lymphocytes (LYMPH, ▨), monocytes (MONO, □) and eosinophils (EOS, ▧) using the murine anti-rat α4 MoAb TA-2, MRα4, the murine anti-human α4 MoAb L25, and the hamster anti-murine β1 MoAb Ha2/5. Data are presented as fold increase in mean fluorescence intensity (MFI) over IgG or IgM control (n = 6).
