Southern blot analysis of the cyclin E gene. Genomic DNAs were digested with either HindIII or Pst I, and the separated fragments were probed with human cyclin E cDNA. Each number over the lane corresponds to the patient number in Table 2. The applied amount of DNA of lane 5 in HindIII panel was small revealed by etidium bromide staining of the gel. “N” represents DNA from mononuclear cells in normal BM.