Fig. 9.
Immunoprecipitation of FcγRIIc from highly purified NK cells. Lysates generated from biotinylated 2 × 106 PBL, NK cells, or U937 cells were incubated with 41H16-, IV.3-, or mIgG2a-coated sepharose-GAM beads o/n at 4°C, eluted by SDS-PAGE, transferred to PVDP membranes, and incubated with HRP-neutravidin. The bands were detected by ECL. Results represent one experiment out of two performed. The molecular markers are shown on margins.
