Fig. 3.
![Fig. 3. Southern blot analysis to determine the FcγRII isoforms expressed by human NK cells. RT-PCR with the FcγRIIa/c-specific primer pair on three A-NK cell samples, U937 and K562 cell lines (positive control), or Molt4 (negative control) was performed as described. The Southern blots were probed with oligonucleotides specific for all the FcγRII isoforms (RS91-46; [A]), for FcγRIIa (HR; [B] and LR; [C]) and for FcγRIIc (IIB; [D]), and cell-specific transcripts of the expected sizes were detected with each probe.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/7/10.1182_blood.v91.7.2369/3/blod4070803.jpeg?Expires=1724242616&Signature=HhEqiiREW8Dho1SJvreEnR03rHgJYAWt3UcOR0KIIo1b-ijG1uEEley2cyRzZtAAhrzhv9xZ5h2xKQv55DC-Hakj4CiH0Om9dbbySlEDg~p6xu7I-GAA0sS7diGFfFlhmJ~VqvLIpP8pz3PxKbnJAJ53WK1eCqnAXMOgUBBnlg-6jhz3TyLXz4iyVlbfNbYo8z3NjeDd4xUJbkuHfktYlZ8c~928di2imp4apszj1~IGC8cJrTakn5-c7L7evCAvdlsaBkyTEG5zK4B7u7IEgEju9qw-vYNFB8kdBd-pQZB97OkMq7ya1gk7hHhHNAo9zhuhB5Crxm-6TJRpwy538A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Southern blot analysis to determine the FcγRII isoforms expressed by human NK cells. RT-PCR with the FcγRIIa/c-specific primer pair on three A-NK cell samples, U937 and K562 cell lines (positive control), or Molt4 (negative control) was performed as described. The Southern blots were probed with oligonucleotides specific for all the FcγRII isoforms (RS91-46; [A]), for FcγRIIa (HR; [B] and LR; [C]) and for FcγRIIc (IIB; [D]), and cell-specific transcripts of the expected sizes were detected with each probe.
