Fig. 2.
![Fig. 2. Immunoblot analysis of HTLV-II virus lysates after reaction with MoAbs specific for HLA class-I (B9.12.1) or class-II (D1.12 and Tu35). Pellets of purified HTLV-II virions generated from C344 cell line (T) or from BJAB cells (B) were incubated in lysis buffer containing 0.2% sodium dodecyl sulfate (SDS) and equivalent concentrations of extracts, as shown by protein staining, analyzed by immunoblot in a 10% polyacrylamide gel containing 0.1% SDS. Immunoreactivity was visualized by chemiluminescence detection system. The size (in kilodaltons [k], indicated on the right) were determined by comparison with protein markers.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/7/10.1182_blood.v91.7.2296/3/blod4073202.jpeg?Expires=1724234549&Signature=XIbZ-T0DRCXfQj~EED7bNeEwmDXr0~8Kvq3njPVhn4hf0SNZvksed8nkUNnTuo9bgWBl3N8YHsvwfZzl1eqsPvT7sIM2jzw1mRl0GI3mPM6upX9L4qWwlcMPLV1g0B1O4~AfNrpibBZ6x5jLuVCfZDZ8SAwfvM2xTRWYbxVwMdsnolOhQKbLatTn4-y9Zp1OiUE3ftVtw23L162-to51Kl80MXX~wRdVhdvvw22iPAxLOuF1haM0PIR7cVCkpEdq1Jj9Dk0cvRq46GaJfzoUEq1wkj5yvsyLSHai-OuhwU5fuPwC4-f5iIhSVp1~6eS~7D3VE5MwOpONqHCrbDxr1Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Immunoblot analysis of HTLV-II virus lysates after reaction with MoAbs specific for HLA class-I (B9.12.1) or class-II (D1.12 and Tu35). Pellets of purified HTLV-II virions generated from C344 cell line (T) or from BJAB cells (B) were incubated in lysis buffer containing 0.2% sodium dodecyl sulfate (SDS) and equivalent concentrations of extracts, as shown by protein staining, analyzed by immunoblot in a 10% polyacrylamide gel containing 0.1% SDS. Immunoreactivity was visualized by chemiluminescence detection system. The size (in kilodaltons [k], indicated on the right) were determined by comparison with protein markers.
