Fig. 5.
![Fig. 5. Coimmunoprecipitation of p27Kip1 and cdk2. 107 U937 cells were treated with PMA for 48 hours. p27Kip1 was immunoprecipitated (IP-1, IP-2, and IP-3) three times from the same extract. Both the immunoprecipitate and the supernatant either before (Pre-IP supernatant [sup]) or after the immunoprecipitate (Post-IP sup) were run on an SDS polyacrylamide gel and Western blotted with p27 antibody. The Western blot was then stripped and reprobed with cdk2 antibody.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/9/10.1182_blood.v90.9.3430/4/bl_0004f5.jpeg?Expires=1722424168&Signature=SmQ1qLtGp6NjoqAZdJVXX9v6HltjxQI2AjzShde5eKhqZxw2FqI5N7Qtiq151na3kSY5YyflwAAc~q5XbPLlkweh-zEzFNTstOt0I5AYMWjYewk~ekyWM-te7HkVS5gU0JD5OYWAhsfEqm3r8gZCbrL6H-BnTqgDRZeHj2-qXi~f1svSKbbw6dWSyQ4ngpbvgyVkEdXrElA9JLowDVUh42JVaktZrd4jGs2X2RkpbworZCeFjoswpmU-Y2HNESnpoQiTdxPrS8eVcowB16mrnjmQpYWlTpY5BZMJZYCYMhqZIGd6h4ZPH5TRKQtEyX8f3zWJMzTDnxywkrKZofOzOA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Coimmunoprecipitation of p27Kip1 and cdk2. 107 U937 cells were treated with PMA for 48 hours. p27Kip1 was immunoprecipitated (IP-1, IP-2, and IP-3) three times from the same extract. Both the immunoprecipitate and the supernatant either before (Pre-IP supernatant [sup]) or after the immunoprecipitate (Post-IP sup) were run on an SDS polyacrylamide gel and Western blotted with p27 antibody. The Western blot was then stripped and reprobed with cdk2 antibody.
