Fig. 1.
![Fig. 1. Effect of PMA on cdk2 activity and phosphorylation. (A) PMA treatment induces inhibition of cdk2 activity. U937 cells were treated with 200 nmol/L PMA for the indicated times. cdk2 was immunoprecipitated from whole cell lysates (400 μg total protein) and analyzed for histone H1 kinase activity. (B) PMA treatment stimulates the dephosphorylation of cdk2. U937 cells were treated with PMA for the indicated times and labeled with [32P]orthophosphate. Cell lysates containing equivalent cpm were immunoprecipitated with anti-cdk2 antibody. Proteins present in the cdk2 immunoprecipitates were resolved by SDS-PAGE and visualized by autoradiography.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/9/10.1182_blood.v90.9.3430/4/bl_0004f1.jpeg?Expires=1722423909&Signature=fHFk0k0kFkK6OYrJ5LXrEkvSyvbztoO9lhuTyJEHr3elaA8656XhBTgKA1KZxp9Ggd2JToeEsSe44UFbi-ur1~9QUtNnw7aG8NBS35uUdPzJaD-gRe~3bqyjxnGFOrndGCTMZ~PWQ6sZbRdTSdVRXYJ8vT4fHoR5YeuB6jS1~0juREi6tDWPz8aR1GhkVpOdHsXyfrK1GPnc-vkMY1Vruq3T7MyYx3GYBPjgGIGrBWrYJa7H62yQBAgzZWZ7rLMthT1YS~OBgezygar1DVgl9z35c7L8Oim9tr2w4srl4ipKkSWEChvFfj-3B~zz4S8tdFndndHX6euIaQQ0O24mqQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of PMA on cdk2 activity and phosphorylation. (A) PMA treatment induces inhibition of cdk2 activity. U937 cells were treated with 200 nmol/L PMA for the indicated times. cdk2 was immunoprecipitated from whole cell lysates (400 μg total protein) and analyzed for histone H1 kinase activity. (B) PMA treatment stimulates the dephosphorylation of cdk2. U937 cells were treated with PMA for the indicated times and labeled with [32P]orthophosphate. Cell lysates containing equivalent cpm were immunoprecipitated with anti-cdk2 antibody. Proteins present in the cdk2 immunoprecipitates were resolved by SDS-PAGE and visualized by autoradiography.
