Time kinetic expression of IL-8 mRNA from fibrin treated HUVEC. Northern blot analysis of IL-8 mRNA expression (top), and 28S and 18S ribosomal RNA (bottom). Lanes 4, 6, 8, 10, and 12 were RNA from HUVEC with control media for 0.5, 1.0, 2.0, 4.0, and 6.0 hours, respectively. Samples from fibrin (1 mg/mL) treated cells for 0.5, 1, 2, 4, and 6 hours were on lanes 5, 7, 9, 11, and 13, respectively. Endothelial cells treated either with thrombin (lane 2), or with LPS 5 μg/mL (lane 3) for 4 hours, and LPS treated human monocytes (lane 1) were run as controls. The RNA blot analyses are representative of two individual studies.