Fig. 4.
![Fig. 4. Effect of ATGs on CD69, CD25, Fas, and Fas-L surface expression. PBL were cultured in presence of medium alone or ATG no. 95-07 and ATG no. 5 at 10 μg/mL and 100 μg/mL for 3 days. At days 0, 1, 2, and 3, surface expression of CD69, CD25, Fas, and Fas-L was determined by cytofluorometry. In parallel, incorporation of [3H]TdR uptake during the last 8 hours of culture was measured (med 367 ± 41 cpm, ATG no. 5 10 μg/mL 391 ± 23 cpm, ATG no. 5 100 μg/mL 252 ± 26 cpm, ATG no. 95-07 10 μg/mL 532 ± 53 cpm, and ATG no. 95-07 100 μg/mL 11,500 ± 103 cpm). Histograms of Fas-L expression at day 1 are shown. Representative of four experiments with ATG no. 95-07 and of two with ATG no. 5.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/7/10.1182_blood.v91.7.2360/3/blod4071904.jpeg?Expires=1724153900&Signature=dUR1NMSb4Crqjz8~~F7cqGHr9x56MK1JaIofEa4a9fum6Z8gMytz25HYuEAMGjmfcCeL9CgJ94cKSMGnUlfB3MqiU8DjBGh3exoHfTIYZsIirIkGMu7s4IyAw~YNCOei54S6lJW7kBTkHXwXuZPeuLH4uLVKt3~wHL1h1beFKlczdy9UoS3MrzWxeY6HyYq9EY1XNplp3pePMQoQftD3vRIJwggpzoAl0vdmVe11JgqNXgG3wKXI9IvSwJzIS3zPXSSGjR6F0NlCzjfwMmesoC888VvdY3PJolsbJXXqmJdLseECT0bQ6NCRN5cf0zWL-L77yuxNmqzEmY9ksGAiWg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of ATGs on CD69, CD25, Fas, and Fas-L surface expression. PBL were cultured in presence of medium alone or ATG no. 95-07 and ATG no. 5 at 10 μg/mL and 100 μg/mL for 3 days. At days 0, 1, 2, and 3, surface expression of CD69, CD25, Fas, and Fas-L was determined by cytofluorometry. In parallel, incorporation of [3H]TdR uptake during the last 8 hours of culture was measured (med 367 ± 41 cpm, ATG no. 5 10 μg/mL 391 ± 23 cpm, ATG no. 5 100 μg/mL 252 ± 26 cpm, ATG no. 95-07 10 μg/mL 532 ± 53 cpm, and ATG no. 95-07 100 μg/mL 11,500 ± 103 cpm). Histograms of Fas-L expression at day 1 are shown. Representative of four experiments with ATG no. 95-07 and of two with ATG no. 5.
