Fig. 1.
Effect of ATGs on mitochondrial transmembrane potential and on phosphatidylserine expression. PBL were activated for 3 days in presence of PHA (5 μg/mL). After removal of dead cells, medium alone, ATG no. 95-07 (10 μg/mL), or CH11 anti-Fas MoAb (1 μg/mL) was added. After 12 hours, ▵Ψm modifications were evaluated by staining with DiOC6 (3). The expression of phosphatidylserine at the surface membrane was evaluated after 15 hours by measuring annexin-V binding. The percentage of cells with decreased mitochondrial potential membrane or increased expression of phosphatidylserine are indicated for each histogram. Results from one typical experiment among four showing similar percentages.
