Fig. 3.
![Fig. 3. Maturation of dendritic cells induced by MCM is irreversible. GM-CSF/IL-4–treated ER− cells were cultured for 4 days in medium alone [−MCM], two different MCM preparations (MCM A and B), or a combination of cytokines equal to that present in the MCM. On day 11, the cells were washed and analyzed for CD83 expression. Residual cells were returned to culture for 3 more days (day 14) in the absence of further supplementation and analyzed for the retention of CD83. MCM contained the following cytokines: (A) IL-1 (92 ng/mL), IL-6 (1 μg/mL), IFN-α (0.02 ng/mL), and TNF-α (182 ng/mL); (B) IL-1 (20 ng/mL), IL-6 (734 ng/mL), IFN-α (.08 ng/mL), and TNF-α (24 ng/mL).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/9/10.1182_blood.v90.9.3640/4/bl_0044f3.jpeg?Expires=1723278344&Signature=4FBb4n1YkKlbjC0OkKagvB1A0c~Eq8is9ZoDJD4xaIXnqRG1Vj0SCd3Q849PCiYQ3ZhJ5XQ8k9h7mqY5QqDABhL3F1kVXgWdEemCeWuoeo5Gew8Ho2HYdUJH9jA0UYoVcPEcRJzH-QPttfsoOhFm-C7YuCNMnZyRrsIcGNObvB4L4av3H6QsJrKjkgbNvB9SDiszBawPO0PJqCzvufoXRarG4nJI1L2s09iJk0O47-LblFCtW~U2RxMHmVA52CnAnp2NOggGc5Ze0uZxqkCypDDdSBRHQGcFELPL~2RwHIZHrPHSPjVr0DykMCWih~8zuzGUYmwQ2gsyMoRdrVoSgA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Maturation of dendritic cells induced by MCM is irreversible. GM-CSF/IL-4–treated ER− cells were cultured for 4 days in medium alone [−MCM], two different MCM preparations (MCM A and B), or a combination of cytokines equal to that present in the MCM. On day 11, the cells were washed and analyzed for CD83 expression. Residual cells were returned to culture for 3 more days (day 14) in the absence of further supplementation and analyzed for the retention of CD83. MCM contained the following cytokines: (A) IL-1 (92 ng/mL), IL-6 (1 μg/mL), IFN-α (0.02 ng/mL), and TNF-α (182 ng/mL); (B) IL-1 (20 ng/mL), IL-6 (734 ng/mL), IFN-α (.08 ng/mL), and TNF-α (24 ng/mL).
