WEHI78/24 cells bind to inflamed, but not normal PLN HEV. Binding of WEHI78/24 cells to normal (A) and CFA-inflamed (B) PLN HEV was assessed in modified Stamper-Woodruff frozen section assays. WEHI78/24 cells were added to freshly cut frozen sections and incubated for 30 minutes at 5°C under constant rotation. Nonadherent cells were washed away and the sections fixed and stained with thionine as described.14 A lower magnification of the normal lymph node is shown to illustrate lack of binding on multiple HEV.