Inhibition of cell migration by increases in cytokine concentration or heterologous cytokine restimulation. FDCP-mix cells (10,000/well) were inoculated at 10 U/mL IL-3 into 96-well plates precoated with 10 μg/cm2 Fn and incubated for 24 hours in a 15° tilted position before observation was started. Cytokines were added at 100 U/mL as indicated during the observation period. Cell movements were analyzed by video microscopy. For each time point, 20 to 25 cells were randomly selected and cells migrated <25 μm within a 20-minute observation period were determined. Values are means ± SD from duplicate determinations.