Influence of function blocking anti-integrin antibodies on progenitor cell migration and adhesion. FDCP-mix cells (10,000 per well) were seeded on BSA, Fn, or Lam (10 μg/cm2 ) coated surfaces at 100 U/mL IL-3. Shown are the responses of migration assays on horizontal surface (□), of migration on tilted surface (), and of adhesion assays (▪) in % of the controls treated with IgG control antibody. Antibodies used were: 9C10 plus R1-2 (anti α4-integrin), 5H10-27 (anti α5-integrin), EA-1 (anti α6-integrin), 9EG7 (anti β1-integrin). Values are means ± SD from triplicate experiments. * Indicates statistically significant difference from control (P < .05, Student's t-test). Control values were: Fn/even migration, 76 ± 6%; Fn/migration on tilted surface, 51 ± 6 per field; Fn/adhesion, 58 ± 5%; Lam/even migration 66 ± 6%; Lam/migration on tilted surface, 33 ± 4 per field; Lam/adhesion, 48 ± 5%.