Fig. 5.
![Fig. 5. Effect of the antagonist anti-Fas MoAb ZB4 on HLA class I–induced apoptosis. PBL were activated with PHA (5 μg/mL) for 3 days. Viable cells were pre-incubated with the antagonist anti-Fas MoAb ZB4 for 1 hour and then treated with medium, YTH862 (10 μg/mL), MoAb90 (10 μg/mL), or the agonist anti-Fas MoAb CH11 (1 μg/mL) for 20 hours. The percentage of apoptotic cells was determined by fluorescent microscopy after staining with Hoescht 33342. In parallel, inhibition of [3H]TdR uptake during the last 8 hours of culture was measured. Results are expressed as mean ± SD of four independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/9/10.1182_blood.v90.9.3629/4/bl_0035f5.jpeg?Expires=1724235494&Signature=XlBLb-dJJtCxw7odqeB3iw0w9NuFoyUDL7OLrJjXHtRSn83bwa2tugQRcsGcOMaKUvNUzEGjWvMhrumczgbb1y508Rd44IzjQrVfxrWHLOyrot6d4i223F0kSNxuPDfz1srXJ7axKwl82W9lDvK9Gg9lwGSAPqgT9u9hnYGwWeWqQuvsH3zhir-G4IEWUJy3C0B67yML7YdZrdT470Bq2tMECnxQgOzP3NjNT85SMSacAyV~5OSkSDQsk63JyZ3A4QM4B-C-K1L3Pawxn--vIv9PtxSENBuR8xRg8J3hnoXJ7fPW53y7AR02QRYK1O7rGl5trHFkEK5lkznZNFV24g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of the antagonist anti-Fas MoAb ZB4 on HLA class I–induced apoptosis. PBL were activated with PHA (5 μg/mL) for 3 days. Viable cells were pre-incubated with the antagonist anti-Fas MoAb ZB4 for 1 hour and then treated with medium, YTH862 (10 μg/mL), MoAb90 (10 μg/mL), or the agonist anti-Fas MoAb CH11 (1 μg/mL) for 20 hours. The percentage of apoptotic cells was determined by fluorescent microscopy after staining with Hoescht 33342. In parallel, inhibition of [3H]TdR uptake during the last 8 hours of culture was measured. Results are expressed as mean ± SD of four independent experiments.
