Fig. 3.
Effect of anti–HLA class I MoAbs on phosphatidylserine externalization and mitochondrial transmembrane potential. Three-day PHA-activated PBL were treated with control IgG1, MoAb90, YTH862, W6/32, or TP25.99 at 10 μg/mL for 24 hours. Externalization of phosphatidylserine was shown by annexin V-binding and Δψm was evaluated after staining with DiOC6(3) as described in Materials and Methods. The percentage of cells that bind annexin V and that of cells with decreased mitochondrial transmembrane potential is indicated for each hictogram. In these conditions, the percentage of apoptotic cells was not modified in resting PBL.
