Fig. 8.
![Fig. 8. Activation of p21Ras in 32D.C10 transfectants. Serum- and growth factor-deprived cells were labeled with [32P]orthophosphate and subsequently stimulated for 5 minutes at 37°C without factor (−), with G-CSF, or with IL-3. Cells were lysed and p21Ras was collected by immunoprecipitation. Nucleotides bound to p21Ras were eluted and separated by thin-layer chromatography. The positions of the GTP and GDP standards are indicated. The level of p21Ras-GTP, expressed as a percentage of total nucleotide bound to p21Ras (GTP + GDP), is given under each lane.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/6/10.1182_blood.v91.6.1924/2/blod4062008.jpeg?Expires=1724941021&Signature=h-HqrRtvG464cJSzjKQCmx4rPcIRKfBYoC314Yxdm4AhwMUogYv2OJl2t8dbudZDfF6yqgU~D-er0QoyAVEmyIDPqhpzMg3YK4hyrd7cWFFA129L~Yt3XGSpsf2neESEZIqTFk1y929tDFqiPn2d2k3lXUrwZWWP4pXVPTdmNif~1kzZNCO0rJDHGwAP2JoEY1nbxpXmmkrDPLwbHeMBOKvwoHyLsGGdtSfXLdYwMihx87TL8WShr4-yoom1UhIT6CZ04usniaBi4KajySiEbSU4eig-dzCVnhueFMGocfVGXarOyWhlmi9dNoYV70cC8Wi2qammB6qLWrfYPjcvQA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Activation of p21Ras in 32D.C10 transfectants. Serum- and growth factor-deprived cells were labeled with [32P]orthophosphate and subsequently stimulated for 5 minutes at 37°C without factor (−), with G-CSF, or with IL-3. Cells were lysed and p21Ras was collected by immunoprecipitation. Nucleotides bound to p21Ras were eluted and separated by thin-layer chromatography. The positions of the GTP and GDP standards are indicated. The level of p21Ras-GTP, expressed as a percentage of total nucleotide bound to p21Ras (GTP + GDP), is given under each lane.
