Effect of reagents that interfere with plasminogen or fibrinogen binding to platelets. Platelets were preincubated 30 minutes with: Ac-pen-RGDC, CpB, EACA, cytochalasin B (CB) or cytochalasin D (CD) before the addition of plasminogen, S2403 and 5 U/mL thrombin. The samples were assayed for plasmin activity after a 5-hour incubation. The reagents had no effect on plasmin generation by unstimulated platelets. The data shown are the mean values of duplicate determinations from two experiments. The duplicate determinations were within 5% of each other.