Characteristics of rat exosome-bead conjugates. (A) The size/structure of exosome-bead conjugates was analyzed using a Coulter Epics XL. The window set as in the left panel allowed visualization of populations of conjugates with single (s), double (d), and triple (t) sized beads (right panel). The window was then set to measure only fluorescence associated with single-bead complexes. (B) Representative flow cytometric analysis of WGA-FITC labeled exos-beads. Conjugates were incubated with lectin, washed, and FACS analysis was performed before (solid line) and after (dotted line) the addition of detergent. (C) Surface expression of AChE on conjugates (solid line). Background staining was obtained by labeling the exosome-bead conjugates only with GAR-FITC (dotted line).