Fig. 1.
![Fig. 1. Effect of different concentrations of TRAP on the platelet surface expression of GPIb and P-selectin. Orgaran anticoagulated whole blood (+ dRGDW) was incubated (5 minutes, 37°C) with various concentrations of TRAP. After 5 minutes, the blood was fixed in 1% paraformaldehyde, incubated with either MoAb 6.20 (against GPIb [▴]) or CD62 MoAb RUU-SP 2.17 (against P-selectin [•]), and analyzed by flow cytometry. Both the binding of the anti-GPIb MoAb before the addition of TRAP and the maximal binding of the anti-CD62 MoAb was assigned 100 arbitrary units of fluorescence. Data represent a typical experiment.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/7/10.1182_blood.v91.7.2353/3/blod4074001.jpeg?Expires=1724233624&Signature=eqKUmY8s5DnFu7l7t2g9hYwQgW3uYUhwP5TCVivteH2iyDvTS8wV9L7YdjBFVAiFb9V6hWFqs2Zoag0p9fEg8duhHO8C30i9Ec9x1zW7AfELvucL8vPb45-22X2OapkLg3obbxn37k31KhKK6DQhwKHlTEORpL4mM2GS4En90X6fRHR8Ni9YulsWSSpSebxiX05N1YJ~B2g8qvWZ8cR6sKYSb4gadec6Zk468F8z53TQJNAcLnDKOn1g87Zp3iqaYdIHl1uwcXMVy6oqpypU8cvwogB-N772K87iS8530UkITNELguywliOSe4lbu-BfybnfTVNfVEz2uOjOPvKyvQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of different concentrations of TRAP on the platelet surface expression of GPIb and P-selectin. Orgaran anticoagulated whole blood (+ dRGDW) was incubated (5 minutes, 37°C) with various concentrations of TRAP. After 5 minutes, the blood was fixed in 1% paraformaldehyde, incubated with either MoAb 6.20 (against GPIb [▴]) or CD62 MoAb RUU-SP 2.17 (against P-selectin [•]), and analyzed by flow cytometry. Both the binding of the anti-GPIb MoAb before the addition of TRAP and the maximal binding of the anti-CD62 MoAb was assigned 100 arbitrary units of fluorescence. Data represent a typical experiment.
