EBV gene expression in lymphoma tissues assessed by RT-PCR analysis. Total RNA extracted from lymphoma tissues of case 1 (lymph node) and case 2 (spleen), and from control EBV–negative BJAB and EBV–positive B95-8 cell lines, was reverse transcribed and subjected to PCR amplification with specific primers. The amplified products containing α-[³²P]dCTP were electrophoresed through 6% acrylamide Tris-borated EDTA gels, followed by autoradiography. For GAPDH control, the amplified products were electrophoresed through a 1.5% agarose gel prestained by ethidium bromide.