![Fig. 1. Detection of CBFB-MYH11 fusion transcripts by RT-PCR. (A) Agarose gel electrophoresis of C1-M1 primer set amplification products. (B) Hybridization of PCR products with the inv(16) CBFB internal oligoprobe. In both panels, lane numbers 1 through 6 correspond to samples from PN 14, 13, 22, 1, 18, and 23, respectively (Table 1). Lane 7 represents a relapse sample from PN 23. Lane 8 is a negative control (no RNA). Sample from PN 22 (lane 3) displays a prominent, but slightly smaller PCR product than expected for a type A fusion (∼400 bp in [A]) and does not hybridize with the oligoprobe (B). This PCR product did hybridize positively with a 0.8-kb CBFB cDNA probe (not shown). Lane 4 demonstrates a type D fusion; lanes 6 and 7 show a type C fusion.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/6/10.1182_blood.v91.6.1882/2/blod4064601.jpeg?Expires=1724207488&Signature=0Cu7iv8QsZ3OhmstUFMa-eyFghSHJu5EQYL-rBMo3MelrsCnq8Mlub7~4sGsxpxEYOO-uNeR~~MA23PA-wqu77rzJbe-6Ttbbii8OitkKSQaDvNYrGLnkiXBStpqzVMwA6mnhYn~XVDUd-qqnRvK7b6JJTnKJr28A6wv7zWDNLu9k3bBaZZqI24rkbgYMEUthhkVgGC6QOkz8RwDaLrAJVGxJipQ41wIosJMPjJt~i2HLw70~LpV5pdYbTthKzZUGyazhZYwOoaeu4xuKs~FFbxGY4hC4IQrAWWKLRe06DeJ0UTJ9em7QgHhG5~nQM4q2Z3mju4oorZ7W48hxuM45Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Detection of CBFB-MYH11 fusion transcripts by RT-PCR. (A) Agarose gel electrophoresis of C1-M1 primer set amplification products. (B) Hybridization of PCR products with the inv(16) CBFB internal oligoprobe. In both panels, lane numbers 1 through 6 correspond to samples from PN 14, 13, 22, 1, 18, and 23, respectively (Table 1). Lane 7 represents a relapse sample from PN 23. Lane 8 is a negative control (no RNA). Sample from PN 22 (lane 3) displays a prominent, but slightly smaller PCR product than expected for a type A fusion (∼400 bp in [A]) and does not hybridize with the oligoprobe (B). This PCR product did hybridize positively with a 0.8-kb CBFB cDNA probe (not shown). Lane 4 demonstrates a type D fusion; lanes 6 and 7 show a type C fusion.
