Kinetics of CD34⁺Thy-1⁺Lin⁻ cell division and differentiation during 6 days of culture. Comparison between IL-3, IL-6, and LIF and TPO, KL, and FL. (A) PKH26 versus CD34 fluorescence. Live Thy-1⁺ cells are shown in blue and live Thy-1⁻ cells are shown in red. Percentages of PKH^hi and CD34^hi PKH^lo and CD34^lo PKH^lo/− cell subsets are shown for this representative experiment (of 3 experiments). (B) forward versus side scatter. Live Thy-1⁺ cells are shown in blue, and live Thy-1⁻ cells are shown in red.