FACS sort gates based on PKH26 versus CD34 fluorescence. After 6 days of culture of CD34⁺Thy-1⁺Lin⁻ cells in different combinations of cytokines, cells were stained with anti-CD34-FITC. Sort gates shown are on live (PI low) cells. These were set based on the PKH26 profile of live unstimulated control cells. Each cytokine condition required a different tissue for sorting and therefore sort gates varied accordingly.