FACS analysis of CD34⁺Thy-1⁺Lin⁻ cells cultured for 6 days in various cytokine combinations containing FL. Numbers are percentages of cells in each quadrant from a representative experiment. Loss of PKH26 fluorescence indicates cell division. Loss of CD34 expression indicates differentiation. Control cells were cultured overnight (D0) or for 6 days (D6) without cytokines and quadrants were set using live-gated undivided cells. The percentages of undivided cells (UR and UL quadrants) are combined.