Effect of suPAR on the binding of¹²⁵I-Gly158scuPA to vascular cells. (A) The specific binding of ¹²⁵I-Gly158scuPA to HVSMC was determined in the absence and presence of increasing suPAR concentrations as indicated. Binding experiments were performed with untreated cells (•) or with piPLC-treated cells (○). Data represent mean ± SEM (cpm/well) of triplicate wells from a typical experiment. Similar results were obtained in three separate experiments on HVSMC or HUVEC, respectively. (B) The effects of MoAb-13H1 against VN (25 μg/mL) and multimeric VN (20 μg/mL) on the binding of ¹²⁵I-Gly158scuPA to piPLC-treated HVSMC were tested in the absence (hatched bars) or presence (filled bars) of suPAR (1 μg/mL). Data are expressed as percentage of control (mean ± SEM) of three different experiments, where 100% (control) is represented by the specific binding of¹²⁵I-Gly158scuPA in the absence of suPAR. Similar results were obtained in experiments with HUVEC.