Association of Shc with the FLT4 receptor upon VRP stimulation. TCLs from VRP-stimulated HEL-JW cells for the indicated times were immunoprecipitated with anti-Shc antibody or normal rabbit IgG as a negative control. The immune complexes were subjected to immunoblotting analysis with anti-FLT4 antibody (upper panel) or anti-Shc antibody (bottom panel). The changes in the association of Shc with the FLT4 receptor were quantitated based on the densitometry values as indicated (by fold increase).