The effect of TGF-β1 on Jo2-induced apoptosis of murine thymocytes. 1 × 106 freshly isolated thymocytes (A) were cultured for 9 hours in RPMI-1640 supplemented with 10% FCS (B) or in medium supplemented with 30 μg/mL cycloheximide in the absence or presence of 2 μg/mL Jo2 (C and D, respectively) or in cycloheximide-containing medium supplemented with TGF-β1 in the absence and presence of Jo2 (E and F, respectively). Cultures were analyzed for apoptotic cells as described in Materials and Methods. An irrelevant hamster IgG control antibody (2 μg/mL) did not affect apoptosis (data not shown). The Y-axis represents relative cell number and the X-axis represents relative fluorescence index. M1 represents percentage of viable cells. One representative experiment (of three) is shown.
