vWF multimer pattern over time in a normal dog after a single bolus injection of human (A) rvWF (35 RCoF U/kg) and 3 days later of (B) pdvWF (32 RCoF U/kg). Plasma samples were analyzed on 1% and 2% SDS-agarose gel electrophoresis. Multimers visualized using a cross-reacting antidog/antihuman vWF antibody and a noncross-reacting antihuman vWF antibody for the post infusion samples.