Fig. 1.
![Dose-response effect of IL-3 and GM-CSF on DNA synthesis in freshly isolated primary HMØs. Purified MØs were cultured in serum-free macrophage medium alone as a control or in the presence of 5 ng/mL IL-3 and at concentrations of 1, 10, or 20 ng/mL GM-CSF, as indicated. Cells (2.6 × 104) were seeded in each well of a 96-well plate. On different days, as shown in the figure, cells were pulsed with 0.1 μmol/L or 2.5 μCi/mL [3H] thymidine and harvested after 6 hours, and counts were taken. The data presented here are the average of counts from 6 individual wells ± standard error.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/6/10.1182_blood.v91.6.2108/2/blod4061601.jpeg?Expires=1722193711&Signature=aZLI0f5OiTYIqR0i5QmaTozRVbYk9DMwvwGYvgUqUE7R4obEJdjcwP9fgCW-vRIxfccLqVsqUb1KeQX40hmHj1goIgkGGipSK6c-waG4K107o72Pxy2xoozL0HqLWe0ZDYpAZnQ55Ptt-l3J3PtLKTwSSE7MnMDqF~Xuy0RWpxzLONki2hfDwHUVYfjOMbaRF4EgSFaMG7Ok9rbWIfp6604o5N5RxBV1RIYD8Asa4VE9W~5oH~CxtwxjTbXAXjXPcy9-AMt4FVUHckxYJAsiSiXud9OpfuVFWSr5bvSbAwOtpIg2Y81LWZj8Oi6Jkdr9ul8xQ43wDArbM~9sv~zD1A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Dose-response effect of IL-3 and GM-CSF on DNA synthesis in freshly isolated primary HMØs. Purified MØs were cultured in serum-free macrophage medium alone as a control or in the presence of 5 ng/mL IL-3 and at concentrations of 1, 10, or 20 ng/mL GM-CSF, as indicated. Cells (2.6 × 104) were seeded in each well of a 96-well plate. On different days, as shown in the figure, cells were pulsed with 0.1 μmol/L or 2.5 μCi/mL [3H] thymidine and harvested after 6 hours, and counts were taken. The data presented here are the average of counts from 6 individual wells ± standard error.
