Flow-cytometric analysis of the effect of sLe^x and CGP69669A on granulocyte expression of sLe^x. Blood samples drawn from mice immediately before and 2 minutes after injection of either sLe^x (100 mg/kg) or CGP69669A (100 mg/kg) were labeled and gated as described in the Methods. Lack of effect of sLe^x and CGP69669A injections on granulocyte expression of L-selectin is shown in A and B, respectively (Dashed line, negative control staining; solid line + no fill, cells drawn before sLe^x or CGP69669A; solid lines + solid fill, cells drawn 2 minutes after injections of sLe^x or CGP69669A). Positive shedding of L-selectin by granulocyte activation with fMLP (10 μm) is shown in C (dashed line, staining with negative control antibody; solid line + no fill, positive Mel-14 staining; solid line + solid fill, Mel-14 staining following fMLP treatment).