Fig. 8.
Purified Myb protein binds in the c-kitpromoter and can be specifically competed. Three different fragments of the c-kit promoter, with nt positions as indicated, were labeled with γ-32 P and then used as probes in EMSA assays performed with bacterial lysates containing, or not containing,Myb protein. (A) Lanes 3, 8, and 13 contain Mybprotein. Lanes 1, 2, 6, 7, 11, and 12 contain no Myb protein. Lanes 4, 9, and 14 contain 100 times excess of cold Myb-binding site competitor (CD34 promoter). Lanes 5, 10, and 15 contain a competitor oligonucleotide with mutated Myb-binding sites. (B) Lanes 1 and 2, no Myb protein in lysate. Lane 3, lysate withMyb protein. Lane 4, lysate with mutated Myb protein (R1 and R2 partially deleted).
