Fig. 6.
![Fig. 6. Inhibition of intracellular degradation causes intracellular accumulation of DeltaF652/3. Parallel plates of transfected COS-1 monkey cells were labeled at 60 hours posttransfection with [35S]methionine for 30 minutes, chased for 4 hours in the absence (lanes 2, 6, 10, 13, 16, and 19) or presence of increasing amounts of ALLN (lanes 3, 4, 7, 8, 11, 12, 14, 15, 17, 18, 20, and 21). CE and conditioned medium (CM) were harvested and equal proportionate volumes of CE and CM were immunoprecipitated with anti-FVIII specific antibody for analysis by SDS-PAGE. Mock indicates cells that did not receive plasmid DNA. Molecular weight size markers are shown on the left of each CE and CM.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/2/10.1182_blood.v91.2.538/3/blod4023206.jpeg?Expires=1722771736&Signature=Mh8n5ocyI19e1Y0SxhmCNcnicYxnQaKd1YB882QjRLHIfW3XNqnETarawNlHpkqbsqKe8KJSa9RWu0Ym9DTz1AUsK1WG98UtpCIc7~EtzG1a~WPKFCIeOdj0oMk8RnJjEmBh2UArkq6ucTfNIErwmN5l5dS05zo0WblXA5vDNQ8bbF-WbUA7VLduxCojDsHBPWlQWiIiq~XOVk3KbZ~0I5BD65Zm4u93oYLBjwRiSzG6hhZDGnpmxWTw4HCjC1io97Te3L8rhVmvdLt~pZ8X6SElOReqAVzEJp7dupMv8OxV2aKNsdhy19G0B4Dea93BInmF-ZqGwaO~oaJgUg3FBg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Inhibition of intracellular degradation causes intracellular accumulation of DeltaF652/3. Parallel plates of transfected COS-1 monkey cells were labeled at 60 hours posttransfection with [35S]methionine for 30 minutes, chased for 4 hours in the absence (lanes 2, 6, 10, 13, 16, and 19) or presence of increasing amounts of ALLN (lanes 3, 4, 7, 8, 11, 12, 14, 15, 17, 18, 20, and 21). CE and conditioned medium (CM) were harvested and equal proportionate volumes of CE and CM were immunoprecipitated with anti-FVIII specific antibody for analysis by SDS-PAGE. Mock indicates cells that did not receive plasmid DNA. Molecular weight size markers are shown on the left of each CE and CM.
