Fig. 2.
![Fig. 2. Levels of IFNR mRNA expression in HUT78 variants. The levels of IFNR mRNA were assessed by slot blot analysis. Total poly A+ RNA was isolated from HUT78R and HUT78S cells treated with (+) or without (−) IFNα and diluted with 6.25 mol/L formaldehyde/10X SSPE at 65°C. The treated RNA (10 μg) was transferred to nylon filters, UV-cross-linked and probed with human IFNR cDNA probe labeled with 32[P], followed by autoradiography.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/91/2/10.1182_blood.v91.2.570/3/blod4024002.jpeg?Expires=1722785193&Signature=qZa-0OBusL5mtqphHlBonkMjeSB814rJWjtpgDPsLm-4lElzbooN5RSyAjwo3q6FqJ9Q03tY5bQrwUrgAHE9tTwy-gLvvaIwrmmFZaLzsljIFH9uK8-X3sCVemepXzi4XDkLPD3AtwEWm5AuAY7CKv9LDDwoO8jHYikzIgnDrdWa5mqsYy~5csqy-zA5Xr-tJNs860NePG~2fwZN1Q1YFeJV7hKI790og916NSIOpgBKsNlUGDBOTR9~iHv-XFvKl4aQP5DVqnoY2YX2plhUw4Zv5UoNZYWmEV6l-OXYIGaklAAZvfZP5rWYsWtLEZi3lKD5Xow-Q~oFiXKO~aKEdA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Levels of IFNR mRNA expression in HUT78 variants. The levels of IFNR mRNA were assessed by slot blot analysis. Total poly A+ RNA was isolated from HUT78R and HUT78S cells treated with (+) or without (−) IFNα and diluted with 6.25 mol/L formaldehyde/10X SSPE at 65°C. The treated RNA (10 μg) was transferred to nylon filters, UV-cross-linked and probed with human IFNR cDNA probe labeled with 32[P], followed by autoradiography.
